diff --git a/langfuse/_client/client.py b/langfuse/_client/client.py
index 6ce72d27a..e03e79f40 100644
--- a/langfuse/_client/client.py
+++ b/langfuse/_client/client.py
@@ -2877,9 +2877,10 @@ async def _process_experiment_item(
                 )
 
                 final_observation_metadata = {
+                    **(item_metadata if isinstance(item_metadata, dict) else {}),
+                    **(experiment_metadata or {}),
                     "experiment_name": experiment_name,
                     "experiment_run_name": experiment_run_name,
-                    **(experiment_metadata or {}),
                 }
 
                 trace_id = span.trace_id
@@ -2920,9 +2921,6 @@ async def _process_experiment_item(
                         {"dataset_id": dataset_id, "dataset_item_id": dataset_item_id}
                     )
 
-                if isinstance(item_metadata, dict):
-                    final_observation_metadata.update(item_metadata)
-
                 experiment_id = dataset_run_id or fallback_experiment_id
                 experiment_item_id = (
                     dataset_item_id or get_sha256_hash_hex(_serialize(input_data))[:16]
diff --git a/tests/unit/test_propagate_attributes.py b/tests/unit/test_propagate_attributes.py
index 0ef92ac10..faa992878 100644
--- a/tests/unit/test_propagate_attributes.py
+++ b/tests/unit/test_propagate_attributes.py
@@ -2748,6 +2748,40 @@ def test_experiment_id_is_stable_across_local_items(
         assert len(experiment_ids) == 1
         assert result.experiment_id == next(iter(experiment_ids))
 
+    def test_experiment_run_metadata_overrides_item_metadata(
+        self, langfuse_client, memory_exporter
+    ):
+        langfuse_client.run_experiment(
+            name="Metadata precedence",
+            run_name="run-name",
+            data=[
+                {
+                    "input": "test",
+                    "metadata": {"shared": "item", "item_only": "yes"},
+                }
+            ],
+            task=lambda *, item, **kwargs: "result",
+            metadata={"shared": "run"},
+        )
+        langfuse_client.flush()
+
+        span = self.get_span_by_name(memory_exporter, "experiment-item-run")
+        self.verify_span_attribute(
+            span,
+            f"{LangfuseOtelSpanAttributes.OBSERVATION_METADATA}.shared",
+            "run",
+        )
+        self.verify_span_attribute(
+            span,
+            f"{LangfuseOtelSpanAttributes.OBSERVATION_METADATA}.experiment_run_name",
+            "run-name",
+        )
+        self.verify_span_attribute(
+            span,
+            f"{LangfuseOtelSpanAttributes.OBSERVATION_METADATA}.item_only",
+            "yes",
+        )
+
     def test_experiment_attributes_propagate_with_dataset(
         self, langfuse_client, memory_exporter, monkeypatch
     ):