diff --git a/Lib/test/test_os.py b/Lib/test/test_os.py
index 555c85f4857c2d7..f4953ef4faed35e 100644
--- a/Lib/test/test_os.py
+++ b/Lib/test/test_os.py
@@ -4042,7 +4042,6 @@ async def test_trailers(self):
     @requires_headers_trailers
     @requires_32b
     async def test_headers_overflow_32bits(self):
-        self.server.handler_instance.accumulate = False
         with self.assertRaises(OSError) as cm:
             await self.async_sendfile(self.sockno, self.fileno, 0, 0,
                                       headers=[b"x" * 2**16] * 2**15)
@@ -4051,7 +4050,6 @@ async def test_headers_overflow_32bits(self):
     @requires_headers_trailers
     @requires_32b
     async def test_trailers_overflow_32bits(self):
-        self.server.handler_instance.accumulate = False
         with self.assertRaises(OSError) as cm:
             await self.async_sendfile(self.sockno, self.fileno, 0, 0,
                                       trailers=[b"x" * 2**16] * 2**15)